Qualified DOT & Non DOT Drug and Alcohol Collection Services

Comprehensive Drug Free Workplace Management Solutions and 24/7 Mobile + In Person Collections.

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$99 DOT Testing Special

Limited-time DOT drug testing offer for CDL drivers. Contact us for full details and availability.

Call Now: (239) 994-5030 Ask About $99 Special

Our Services

DOT Collections

Qualified drug and alcohol collections following 49 CFR part 40, including urine and BAT testing. Mobile + In Person service available. Oral fluid testing coming June 2025.

Non-DOT Testing

Comprehensive non-DOT testing options including rapid oral fluid tests with instant results, plus laboratory-based urine, hair, and blood testing services.

TPA & Consulting Services

Expert guidance for drug-free workplace policies under state and federal laws, including DOT/FMCSA compliance. Custom consulting packages with Mobile + In Person support available.

DNA Paternity Testing

Professional DNA paternity testing services with confidential results. We provide accurate, legally admissible testing with quick turnaround times and secure handling of all samples.

Immediate Testing

Fast-response testing services available for urgent situations. Get rapid results when you need them most, with our Mobile + In Person collection capabilities and expedited processing options.

Mobile + In Person Collection Services

On-site and clinic-based testing for all collection types including pre-employment, random, post-accident, reasonable suspicion, return-to-duty, and follow-up testing requirements.

Counseling & Evaluations

Telehealth substance abuse evaluations, group counseling, and coordinated drug and alcohol screenings with a Master Certified Addiction Professional.

Learn more about counseling services

Benefits of Mobile + In Person Testing

  • On-site testing eliminates employee travel time
  • Reduces time away from work duties
  • Testing available during any shift for 24/7 operations

  • Flexible scheduling around your business operations
  • Multiple employees tested in succession at your location
  • Reduced administrative coordination burden

  • Service available at multiple job sites or locations
  • Perfect for remote work locations and construction sites
  • Ideal for companies with distributed workforce

  • Professional testing environment at your location
  • Maintained chain of custody procedures

  • Rapid response for post-accident testing
  • Available for reasonable suspicion situations
  • Quick mobilization for random testing programs
About Us

About Us

Straight Line Mobile Testing & Lab Services is your reliable partner for drug and alcohol testing throughout Lee County and beyond. As a DOT-qualified collector, we offer both regulated and non-regulated testing—on-site and in person at clinic locations—with 24/7 Mobile + In Person services tailored for clients who can’t access a lab.

Our experienced, bilingual team (English & Spanish) combines professionalism with convenience, using integrated software to locate labs, send orders, and receive results electronically. We also offer nationwide testing access and full-service C/TPA support, including DOT-compliant random pool management.

Whether you need immediate testing or a fully managed workplace program, we deliver confidential, dependable service—right where you are.

No time wasted, we come straight to you.

Why Choose Us

Mobile Service

On-site testing services at your location for maximum convenience.

DOT Qualified

Fully Qualified collector for all DOT-mandated testing requirements.

Comprehensive Testing

Multiple testing methods including urine, hair, oral fluid, and BAT.

Expert Team

Professional guidance for drug-free workplace programs.

What Our Clients Say

"Exceptional service and professional staff. They guided me through the entire CDL reinstatement process with expertise and patience. Highly recommended!"

- Robert Anderson, Professional Driver

"Fast, reliable, and incredibly helpful. They understood the urgency of getting back on the road and made the process smooth and stress-free."

- Maria Rodriguez, Owner-Operator

"Their expertise in CDL reinstatement was invaluable. They handled everything professionally and got me back to work quickly. Couldn't ask for better service!"

- James Thompson, Long-haul Driver

Counseling & Substance Abuse Evaluations

Telehealth & in-person evaluations, group counseling, and coordinated screenings with a Master Certified Addiction Professional.

Substance Abuse Evaluations

Court, probation, employer, and DOT-related referrals, with clear recommendations and documentation for program requirements.

Group Counseling Sessions

Structured education and support groups focused on understanding substance use, decision-making, and meeting referral expectations.

Drug & Alcohol Screenings

Testing options coordinated with counseling and evaluation services, aligned with what courts, employers, and programs require.

Ready to schedule a counseling session or evaluation, or need more details about requirements?

Learn More About Counseling Ask About Counseling & Evaluations

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Need immediate testing or other services?

Contact us now for rapid response testing services

(239) 994-5030